A Controlled LC-SICRIT-MS Method for Structural Elucidation of Double-Bonds in Unsaturated Lipids by MS1
A novel, tunable LC-SICRIT-MS method is developed to provide double-bond structural elucidation of lipids by exploiting the inherent formation of oxidative species from secondary reactive species generated in the plasma. This leads to oxidative cleavage of the analyte's double bond, enabling MS1 analysis without additives or external ozone as commonly required in the collision chamber of a triple quad MS.
Concept
Here, a method was developed using the plasma-based ionization technology SICRIT to generate the respective reactive species directly. With this method, the LC method itself can be used to generate reactive oxygen species within the ionization source. This leads to unambiguous double bond cleavage for MS1 analysis of the respective species. Not only is this method applicable for the typical LC-MS/MS set-up, but with the ability to also conduct direct analysis with the source, it is possible to observe MS1 oxidative cleavage under aqueous solvent conditions.
This gas-phase ionization technique can conduct controlled, immediate, oxidative cleavage without dopants or ozone generators. Additionally, this technique can be used in a direct analysis configuration to perform the oxidative cleavage for the rapid screening of particular fragment masses. With this approach, we present here a universal source for controlled oxidative cleavage, available in both chromatographic and direct configurations for lipidomic and metabolomic studies.
Responsible
Ciara Conway
Funding
Plasmion GmbH