DetectRespi

Objectives:

The demand of so-called point-of-care (PoC) devices to carry out diagnostics fast and on site is increasing. Currently, there are only a few PoC systems that work based on molecular biology. These systems will be necessary in the future for an early detection of infections.

Thus, the aim of the project DetectRespi is the development of a molecular biological Lab-on-a-chip platform for the highly parallel detection of viral and bacterial pathogens of respiratory diseases. The molecular biological basis will be a multiplex PCR coupled to a DNA microarray. Main tasks for the biochemical assay are the preparation of primers and probes for the panel and the development and preparation of the individual assay steps as well as the implementation into a multiplex PCR. The evaluation of the DNA array will be done by fluorescence labeling of the PCR products using Cy5. To achieve this, an optical readout unit will be designed, realized, and integrated in the final device.

Responsible:

Eva Krois (eva.krois@tum.de)
Amelie Hohensee (amelie.hohensee@tum.de)

Funding:

AiF-ZIM

Partners:

Mildendo
M2 Automation
Tropeninstitut KUM
BIOMANGUINHOS
IBMP