FluRam

Objectives:

High performance liquid chromatography (HPLC) is an analytical separation method. Detectors coupled at the end of the column of the HPLC system are the essential link to provide measurable analytical information of the chromatographic separation.

The Raman spectroscopy is an attractive optical spectroscopy technique for potential future detector systems. The technique provides a "molecular fingerprint" of a sample and allows non-destructive analysis of chemical composition and molecular structure via in-line analysis.

The objective of the project is the development of a novel high performance liquid chromatography detector for automated analysis of HPLC fractions by molecule specific light scattering. Based on Raman spectroscopic methods, analysis with high selectivity and sensitivity will be performed in common eluent gradients between water and methanol, ethanol, acetonitrile or isopropanol at flow rates of about 1 mL/min. A flow cell and powerful excitation and detection systems will be developed to detect Raman-active analytes at concentrations of 0.001-100 ppm in microfluidic flow systems. A measurement and evaluation scheme at various flow rates for different eluents will be developed for coupling the detector to an HPLC system. The Raman spectroscopic flow system will be designed for HPLC systems, however, it will be designed in such a way that it can be used manifold for process analysis in the field of chemistry and pharmacy for the detection of different substances.

Responsible:

Lucas Hirschberger (lucas.hirschberger@tum.de)

Funding:

AiF-ZIM

Partners:

Soliton GmbH
Solectrix GmbH