Tuberculosis is an infectious disease that can be transmitted through the air and is one of the most common causes of death worldwide. Although there are drugs against tuberculosis, they have many side effects. Furthermore, mycobacteria can shut down their metabolism, so the currently recommended treatment extends over four to six months.

From the analysis of a present RNA from mycobacteria at a certain point in time, the respective rate of new formation cannot be inferred, since the half-life of the RNA seems to change depending on the growth state. Currently, there is no suitable technique for mapping RNA dynamics. This will be the aim of this project. For this purpose, different coding and non-coding sequences will be hybridized on a chip, which will be read out by mass spectrometry. It will be investigated how the mRNA synthesis rate correlates with the protein formation rate and how antimycobacterial products influence the mRNA half-life.